Coding

Part:BBa_C2008:Design

Designed by: Reshma Shetty   Group: iGEM07_Example   (2007-08-22)


MBP.p53ZF F2F3.GCN4 homodimeric repressor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 360
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 58


Design Notes

Two silent mutations were introduced to remove an internal NotI site and a rare arginine codon.


Source

This coding region was rationally designed based on BBa_C2006 and p53ZF.

References